s40064-016-1861-x

Recombinant Human Macrophage Inflammatory Protein 1 alpha Protein Summary

    Description
    A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 24-92 of Human Macrophage Inflammatory Protein 1 alpha

    Source: E.coli

    Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA

    Source
    E. coli
    Protein/Peptide Type
    Recombinant Protein
    Gene
    CCL3
    Purity
    >90%, by SDS-PAGE
    Endotoxin Note
    < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

    Theoretical MW
    10 kDa.
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

    Storage
    Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
    Buffer
    10mM Sodium Citrate (pH3.5) containing 10% glycerol
    Preservative
    No Preservative
    Concentration
    0.25 mg/ml
    Purity
    >90%, by SDS-PAGE

Alternate Names for Recombinant Human Macrophage Inflammatory Protein 1 alpha Protein

      C-C motif chemokine 3
      chemokine (C-C motif) ligand 3
      G0S19-1Tonsillar lymphocyte LD78 alpha protein
      LD78ALPHA
      Macrophage inflammatory protein 1-alpha
      MIP1AG0/G1 switch regulatory protein 19-1
      MIP-1-alphaSIS-beta
      SCYA3PAT 464.1
      small inducible cytokine A3 (homologous to mouse Mip-1a)
      Small-inducible cytokine A3

Background

CCL3, also known as, macrophage inflammatory protein-1alpha (MIP-1alpha), is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This protein has been identified as one of the major HIV-SFs produced by CD8+ T cells, along with MIP-1beta and RANTES. It acts as an inhibitor of different strains of HIV-1, HIV-2 and SIV infection in a dose-dependent manner. Recombinant human CCL3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

product targets : SPHK inhibitors

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