journal.pone.0145404

Recombinant Human IL-7 Protein Summary

    Description
    Recombinant bioactive protein containing 153 amino acids for Human IL-7

    Source:E. coli
    Amino Acid Sequence:(P13232 )

    Preparation
    Method
    >98% SDS-PAGE
    Details of Functionality
    The ED50 as determined by the dose-dependent stimulation of the proliferation of murine 2E8 cells is <0.5 ng/ml, corresponding to a specific activity of >2 x 106 units/mg.
    Source
    E. coli
    Protein/Peptide Type
    Biologically Active Protein
    Gene
    IL7
    Purity
    >95%, by SDS-PAGE
    Endotoxin Note
    <0.1 ng/ug

Applications/Dilutions

    Theoretical MW
    17.4 kDa.
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

    Storage
    Store at -20 to -80C. Avoid freeze-thaw cycles.
    Buffer
    Lyophilized from 10 mM HoAc
    Concentration
    Lyoph
    Purity
    >95%, by SDS-PAGE
    Reconstitution Instructions
    Reconstitute in H2O to a concentration of 0.1 mg/ml, pipet up-and-down to dissolve. The solution can be diluted into other buffered solutions or stored at -20C for future use.

Alternate Names for Recombinant Human IL-7 Protein

      IL7
      IL-7
      IL-7interleukin-7
      interleukin 7
      Lymphopoietin-1
      PBGF

Background

IL-7 is a hematopoietic growth factor which affects primarily early B and T cells. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as ConA and IL-2. Human and murine IL-7 is cross-species reactive. Recombinant human IL-7 is a 17.4 kDa protein containing 153 amino acid residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

VX-810

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