journal.pone.0145000

Recombinant Human IL-21 Protein Summary

    Description
    Recombinant bioactive protein containing 133 amino acids for Human IL-21

    Source:E. coli
    Amino Acid Sequence:(Q9HBE4)

    Details of Functionality
    The recombinant human IL-21 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of human peripheral blood mononuclear cells is <5 ng/ml.
    Source
    E. coli
    Protein/Peptide Type
    Biologically Active Protein
    Gene
    IL21
    Purity
    >90%, by SDS-PAGE
    Endotoxin Note
    <0.1 ng/ug

Applications/Dilutions

    Theoretical MW
    15.5 kDa.
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

    Storage
    Store at -20 to -80C. Avoid freeze-thaw cycles.
    Buffer
    Lyophilized from solution containing no additives.
    Concentration
    Lyoph
    Purity
    >90%, by SDS-PAGE
    Reconstitution Instructions
    Reconstitute in H2O to a concentration of 0.1-1.0 ug/ul (Note: Gently pippetting to dissolve). The solution can be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use.

Alternate Names for Recombinant Human IL-21 Protein

      IL21
      IL-21
      IL-21Za11interleukin-21
      interleukin 21
      interleukin-21 isoform

Background

Recombinant human IL-21 is a homodimeric, non-glycosylated polypeptide containing 133 amino acid residues and has a molecular weight of 15.5 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

CGS 21681 (Hydrochloride)

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