Recombinant Human IGF-I Protein Summary
Recombinant bioactive protein for Human IGF-I
Source:E. coli
Amino Acid Sequence:(P01343)
Method
>97% SDS-PAGE
The ED50 as determined by a cell proliferation assay using NIH3T3 cells is typically 1-6 ng/ml.
E. coli
Biologically Active Protein
IGF1
>95%, by SDS-PAGE
<0.1 ng/ug
Applications/Dilutions
9.11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -20 to -80C. Avoid freeze-thaw cycles.
Lyophilized with no additives
Lyoph
>95%, by SDS-PAGE
Reconstitute in dH2O or 10 mM AcOH to 1.0 mg/ml. This solution can then be diluted into other buffered solutions or stored at 4C for 1 week or -20C for future use.
Alternate Names for Recombinant Human IGF-I Protein
- IBP1
- IGF1
- IGF-1
- IGF1A
- IGFI
- IGF-I
- IGF-IA
- IGF-IB
- insulin-like growth factor 1 (somatomedin C)
- insulin-like growth factor 1
- insulin-like growth factor I
- insulin-like growth factor IA
- insulin-like growth factor IB
- Mechano growth factor
- MGF
- Somatomedin A
- Somatomedin C
- somatomedin-C
Background
IGF-I (insulin-like Growth Factor-I) is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. s recombinant human IGF-I is a N-terminus modified IGF-I with a Long R3 chain, which shows higher potency than regular human IGF-I in bioactivity assay. The recombinant IGF-I Long R3 was produced from E. coli using Animal Origin Free (AOF) components and therefore it is suitable for bioproduction also. The product can be provided as lyophilized form or liquid form, stable for at least two years.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.