Recombinant Human Glutathione Peroxidase 1/GPX1 Protein Summary
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-203 of Human Glutathione Peroxidase 1/GPX1
Source: E.coli
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MCAARLAAAA AAAQSVYAFS ARPLAGGEPV SLGSLRGKVL LIENVASLCG TTVRDYTQMN ELQRRLGPRG LVVLGFPCNQ FGHQENAKNE EILNSLKYVR PGGGFEPNFM LFEKCEVNGA GAHPLFAFLR EALPAPSDDA TALMTDPKLI TWSPVCRNDV AWNFEKFLVG PDGVPLRRYS RRFQTIDIEP DIEALLSQGP SCA
E. coli
Recombinant Protein
GPX1
>90%, by SDS-PAGE
Applications/Dilutions
24.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 30% glycerol, 100mM NaCl
No Preservative
0.5 mg/ml
>90%, by SDS-PAGE
Alternate Names for Recombinant Human Glutathione Peroxidase 1/GPX1 Protein
- Cellular glutathione peroxidase
- CGPx
- EC 1.11.1
- EC 1.11.1.9
- Glutathione Peroxidase 1
- GPX1
- GPx-1
- GSHPX1
- GSHPx-1
- MGC14399
- MGC88245
Background
GPX1 (Glutathione peroxidase 1) belongs to the glutathione peroxidase family, consisting of eight known glutathione peroxidases (Gpx1-8) in humans. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. The GPX1 is part of the enzymatic antioxidant defence, preventing oxidative damage to DNA, proteins and lipids by detoxifying hydrogen and lipid peroxides that may contribute to prostate cancer development. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. Recombinant human GPX1(U49C) protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.
product targets : PKD inhibitors