Recombinant Human Galectin-3 Protein Summary
Recombinant bioactive protein containing 250 amino acids for Human Galectin-3
Source:E. coli
Amino Acid Sequence:(P17931)
Method
>98% SDS-PAGE
Determined by its ability to chemoattract human blood monocytes. Chemotactic activity was observed at a concentration of 2.5 ug/ml with a peak response obtained at 250 ug/ml.
E. coli
Biologically Active Protein
LGALS3
>95%, by SDS-PAGE
<0.1 ng/ug
Applications/Dilutions
Unit Definition: One unit is defined as the amount of enzyme that hydrolyzes 1 nmol/min of Ac-IETD-PNA per min at 25oC in 50 mM Tris pH 8.0 containing 150 mM NaCl and 0.01% Triton X-100.
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -20 to -80C. Avoid freeze-thaw cycles.
Sterile filtered and lyophilized from 0.5X PBS, pH 7.2.
Lyoph
>95%, by SDS-PAGE
Reconstitute in H2O to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use.
Alternate Names for Recombinant Human Galectin-3 Protein
- AGE-R3
- Carbohydrate-binding protein 35
- CBP 35,35 kDa lectin
- CBP35
- GAL3
- Gal-3
- galactin-3
- Galactose-specific lectin 3
- Galactoside-binding protein
- GALBPCBP35
- Galectin3
- Galectin-3
- GALIG
- IgE-binding protein
- L29
- L31
- L-31
- Laminin-binding protein
- Lectin L-29
- lectin, galactoside-binding, soluble, 3
- LGALS2
- LGALS3
- Mac-2 antigen
- Mac-2
- MAC2GAL3
Background
Lectins are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with Beta -galactoside moieties. Galactin-3 regulates a number of biological processes, including embryogenesis, inflammatory responses, cell progression and metastasis. Galectin-3 can function intraCellularly in controlling cell cycle and preventing T-cell apoptosis, and also extraCellularly, in activating various cells, including monocytes/macrophages, mast cells, neutrophils, and lymphocytes. Recombinant Galectin-3 is a globular 26.0 kDa protein containing 250 amino acid residues, but no disulfide bonds.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.