Recombinant Human FGF basic/FGF2 Protein Summary
Recombinant bioactive protein containing 147 amino acids for Human FGF basic/FGF2
Source:E. coli
Amino Acid Sequence:(P09038) – MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS
Method
>97% SDS-PAGE
EC50 is typically 0.67-1.45 ng/ml. The activity was determined by the dose-dependent stimulation of mouse BALB/c 3T3 cells.
E. coli
Biologically Active Protein
FGF2
>95%, by SDS-PAGE
<0.01 ng/ug
Applications/Dilutions
16.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -20 to -80C. Avoid freeze-thaw cycles.
Lyophilized from a 10 mM Na2PO4, pH 8.0.
Lyoph
>95%, by SDS-PAGE
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Alternate Names for Recombinant Human FGF basic/FGF2 Protein
- basic fibroblast growth factor bFGF
- Basic fibroblast growth factor
- bFGF
- FGF basic
- FGF2
- FGF-2
- FGF2AS
- FGFBprostatropin
- fibroblast growth factor 2 (basic)
- GFG1
- HBGF-2
- HBGH-2
- heparin-binding growth factor 2
- NUDT6
- Prostatropin
Background
Fibroblast Growth Factors, FGFs, are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. Although the mechanism remains unclear, FGF-basic 147 (variant of FGF basic 154) is a critical component in keeping embryonic stem cells undifferentiated in cell culture systems. Recombinant human FGF-b 147 (FGF-2) is a non-glycosylated protein, containing 147 amino acids, and having a molecular mass of 16.5 kDa.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.
Dorsomorphin (dihydrochloride)