Recombinant Human Erythropoietin Protein Summary
Recombinant bioactive protein containing 166 amino acids for Human Erythropoietin
Source:CHO cells
Amino Acid Sequence:(P01588)
Method
>98% SDS-PAGE
Determined by a cell proliferation assay using TF-1 cells. The expected ED50 for this effect is 0.8-1.0 ng/ml.
CHO
Biologically Active Protein
EPO
>95%, by SDS-PAGE
<0.1 ng/ug
Applications/Dilutions
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -20 to -80C. Avoid freeze-thaw cycles.
lyophilized in sodium citrate buffer containing sodium chloride
Lyoph
>95%, by SDS-PAGE
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1 to 1.0 mg/ml. This solution can then be diluted into other aqueous buffers containing a carrier protein (like 0.1% BSA) and stored in working aliquots at -20C to -80C for future use.
Alternate Names for Recombinant Human Erythropoietin Protein
- EP
- EPO
- epoetin
- Erythropoietin
- MGC138142
- MVCD2
Background
Recombinant Human Erythropoietin-alpha produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain containing 165 amino acids and having a predicted molecular mass of 21 kDa and apparent glycosylated molecular mass of 30.4 kDa. EPO-alpha is purified by proprietary chromatographic techniques.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.