Recombinant Human Endostatin Protein Summary
Recombinant bioactive protein for Human Endostatin
Source:Pichia Pastoria
Amino Acid Sequence:(P39060)
Method
>98% SDS-PAGE
human Endostatin is biologically active when compared to standards. The activity as calculated by ECE migration inhibition was found to be 50,000 IU/mg.
Biologically Active Protein
COL18A1
>95%, by SDS-PAGE
<0.1 ng/ug
Applications/Dilutions
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -20 to -80C. Avoid freeze-thaw cycles.
Sterile filtered liquid
Lyoph
>95%, by SDS-PAGE
Alternate Names for Recombinant Human Endostatin Protein
- antiangiogenic agent
- COL18A1
- collagen alpha-1(XVIII) chain
- collagen, type XVIII, alpha 1
- Endostatin
- FLJ27325
- FLJ34914
- human type XVIII collagen10endostatin
- KNO
- KNO1MGC74745
- Knobloch syndrome, type 1
- KS
- multi-functional protein MFP
Background
Endostatin has been identified as a C-terminal fragment of Collagen type 18, a recently identified member of a family of collagen-like proteins referred to as multiplexin family Endostatin specifically inhibits proliferation of endothelial cells although it does not affect the proliferation of EOMA cells. Endostatin also potently inhibits angiogenesis and tumor growth. Endostatin has an important role in endothelial cell adhesion and cytoskeletal organization. Endostatin can be found in vessel walls (elastic fibers) and basement membranes. Recombinant Endosatin expressed in yeast causes G1 arrest of endothelial cells, and endostatin treatment results in apoptosis of HUVE and HMVE cells.DESCRIPTION: Recombinant Human Endostatin produced in Pichia Pastoris is a single, glycosylated, polypeptide having a total molecular mass of 20,000 Dalton C-terminal fragment of collagen XVIII that has been shown to act as a potent inhibitor of angiogenesis and tumor growth in vitro and in vivo. Induces tyrosine phosphorylation of Shc (SH2 domain adapter protein) leading to specific inhibition in endothelial cell proliferation.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.