Recombinant Human Elastase Protein Summary
Native Elastase
Source:Human Neutrophil
Amino Acid Sequence:(P08246)
Biologically Active Protein
ELANE
>95%, by SDS-PAGE
Applications/Dilutions
Specific activity: 20-22 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one micromole of Meo-Suc-Ala-Pro-Val-pNA per minute at 25C in 100 mM Tris-HCl, pH 7.5, with 500 mM NaCl.
29.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -20 to -80C. Avoid freeze-thaw cycles.
Salt- free lyophilized solid
Lyoph
>95%, by SDS-PAGE
Reconstitute with deionized H2O
Alternate Names for Recombinant Human Elastase Protein
- CBPP
- cholesterol-binding pancreatic protease
- chymotrypsin-like elastase family member 3B
- chymotrypsin-like elastase family, member 3B
- E1
- EC 3.4.21
- EC 3.4.21.70
- EL-1
- ELA3B
- elastase 1
- elastase 3B, pancreatic
- Elastase IIIB
- elastase-3B
- fecal elastase 1
- pancreatic elastase 1
- pancreatic endopeptidase E
- Protease E
- proteinase E
Background
Elastase, like trypsin and chymotrypsin, is a serine protease that also hydrolyses amides and esters. It is produced in the pancreas as an inactive zymogen, proelastase, and activated in the duodenum by trypsin. Elastase is distinctive in that it acts upon elastin.This enzyme degrades elastin, collagen, and proteoglycan and has been implicated in the development of pulmonary emphesema and rheumatoid arthritis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.