Recombinant Human EEF1E1 Protein Summary
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-174 of Human EEF1E1
Source: E.coli
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAAAAELSLL EKSLGLSKGN KYSAQGERQI PVLQTNNGPS LTGLTTIAAH LVKQANKEYL LGSTAEEKAI VQQWLEYRVT QVDGHSSKND IHTLLKDLNS YLEDKVYLTG YNFTLADILL YYGLHRFIVD LTVQEKEKYL NVSRWFCHIQ HYPGIRQHLS SVVFIKNRLY TNSH
E. coli
Recombinant Protein
EEF1E1
>95% pure by SDS-PAGE
Applications/Dilutions
21.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
No Preservative
1 mg/ml
>95% pure by SDS-PAGE
Alternate Names for Recombinant Human EEF1E1 Protein
- AIMP3Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3
- ARS-interacting multifunctional protein 3
- Elongation factor p18
- eukaryotic translation elongation factor 1 epsilon 1
- eukaryotic translation elongation factor 1 epsilon-1
- Multisynthase complex auxiliary component p18
- p18 component of aminoacyl-tRNA synthetase complex
- P18
Background
EEF1E1, also known as, eukaryotic translation elongation factor 1 epsilon-1, shares sequence similarity with the amino-terminal ends of the Beta and Gamma subunits of EF-1. By specifically interacting with MetRS, this protein binds to a macromolecular tRNA synthtase complex that catalyzes the ligation of specific amino acids to their cognate tRNAs. Upon DNA damage, it translocates to the nucleus where it interacts with ATM and ATR, resulting in p53 activation. Recombinant human EEIF1E1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.
product targets :　Metabolic Enzyme_Protease inhibitors