Recombinant Human Defensin beta 1 Protein Summary
Recombinant bioactive protein containing 47 amino acids for Human Defensin beta 1
Source:E. coli
Amino Acid Sequence:(P60022)
Method
>98% SDS-PAGE
Determined by its ability to chemoattract CD34+ dendritic cells using a concentration range of 0.1-1 ug/ml.
E. coli
Biologically Active Protein
DEFB1
>95%, by SDS-PAGE
<0.1 ng/ug
Applications/Dilutions
5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -20 to -80C. Avoid freeze-thaw cycles.
Lyophilized from a concentrated solution containing 20 mM PBS pH-7.4 and 130 mM sodium chloride
Lyoph
>95%, by SDS-PAGE
Reconstitute in dH2O to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use.
Alternate Names for Recombinant Human Defensin beta 1 Protein
- BD1
- BD-1
- beta-defensin 1
- beta-defensin-1
- DEFB-1
- DEFB101
- defensin, beta 1HBD-1
- hBD-1
- HBD1MGC51822
Background
The Defensin family are highly similar in their protein sequence and are microbicidal & cytotoxic peptides made by neutrophils. Beta Defensin-1 (BD-1) is an antimicrobial peptide having the resistance of epithelial surfaces to microbial colonization. BD-1 has close proximity to Defensinalpha-1 and has been implicated in the pathogenesis of cystic fibrosis. Skin of patients having atopic dermatitis patients and mycosis fungoides (non-lesional and lesional) show lower human BD-1 mRNA expression and higher human BD-2 and human BD-3 mRNA expression. Beta Defensin is highly expressed by epithelial cells. BD-1 may play a role in the pathogenesis of severe sepsis. Variation in human BD-1 contributes to asthma diagnosis, with apparent gender-specific effects. Human BD-3 is a dimer, while Human BD-1 and Human BD-2 are monomeric. Recombinant human Beta Defensin-1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 47 amino acids and having a molecular mass of 5 kDa.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.