Recombinant Human Crk Protein Summary
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-204 of Human Crk
Source: E.coli
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAGNFDSEER SSWYWGRLSR QEAVALLQGQ RHGVFLVRDS STSPGDYVLS VSENSRVSHY IINSSGPRPP VPPSPAQPPP GVSPSRLRIG DQEFDSLPAL LEFYKIHYLD TTTLIEPVSR SRQGSGVILR QEEAEYVRAL FDFNGNDEED LPFKKGDILR IRDKPEEQWW NAEDSEGKRG MIPVPYVEKY RPASASVSAL IGGR
E. coli
Recombinant Protein
CRK
>95% pure by SDS-PAGE
Applications/Dilutions
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
20 mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
No Preservative
0.5 mg/ml
>95% pure by SDS-PAGE
Alternate Names for Recombinant Human Crk Protein
- adapter molecule crk
- avian sarcoma virus CT10 (v-crk) oncogene homolog
- Crk
- CRKII
- p38
- Proto-oncogene c-Crk
- v-crk avian sarcoma virus CT10 oncogene homolog
- v-crk sarcoma virus CT10 oncogene homolog (avian)
Background
CRK is a member of signaling adapter protein family that binds to several tyrosine-phosphorylated proteins. This protein is involved in many cellular processes including apoptosis, proliferation, and differentiation. It has a modular domain architecture consisting of an SH2 followed by two SH3 domains (src-homology domains). The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Recombinant human CRK protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.
product targets : DNA Stain inhibitors