Recombinant Human Caspase-1 Protein Summary
Recombinant protein for Human Caspase-1
Source:E. coli.
Amino Acid Sequence:(NP_150634.1)
E. coli
Biologically Active Protein
CASP1
SDS-PAGE
Applications/Dilutions
Unit Definition: One unit of the recombinant caspase-1 is the enzyme activity that cleaves 1 nmol of the caspase substrate YVAD-pNA (pNA: pnitroanaline) per hour at 37C in a reaction solution containing 50 mM Hepes pH 7.2 50 mM NaCl 0.1% Chaps 10 mM EDTA 5% Glycerol and 10 mM DTT.
Molecular Weight: large (20 kD) and small (10 kD) subunitsk
Specific activity: > 5000 units/mg
Molecular Weight: large (20 kD) and small (10 kD) subunitsk
Specific activity: > 5000 units/mg
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -20 to -80C. Avoid freeze-thaw cycles.
Lyophilized powder
Lyoph
SDS-PAGE
Reconstitute to 1 unit per ul in PBS containing 15% glycerol.
Alternate Names for Recombinant Human Caspase-1 Protein
- CASP1
- CASP-1
- caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta
- caspase 1, apoptosis-related cysteine protease (interleukin 1, beta
- Caspase1
- Caspase-1
- EC 3.4.22
- EC 3.4.22.36
- ICE
- ICEP45
- IL-1 beta-converting enzyme
- IL1BC CASP1 nirs variant 1
- IL-1BC
- IL1BCE
- IL1B-convertase
- interleukin 1-B converting enzyme
- interleukin 1-beta convertase
- Interleukin-1 beta convertase
- Interleukin-1 beta-converting enzyme
- p45
Background
Caspase-1 (also know as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kD) and small (10 kD) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form. The recombinant active human caspase-1 was expressed in E. coli. The active caspase-1 preferentially cleaves caspase-1 substrates (e.g., YVAD-AFC or YVAD-pNA) and is routinely tested at for its ability to enzymatically cleave these two substrates Ac-YVAD-pNA (Cat. #1104-200) or Ac-YVAD-AFC (1103-200). The rh-Caspase has an N-term His-tag and corresponds to amino acids 120-404 of Caspase-1, gene accession# NP_150634.1.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.