Recombinant Human Caspase-10/FLICE2 Protein Summary
Recombinant protein for Human Caspase-10/FLICE2
Source:E. coli.
Amino Acid Sequence:(Q92851)
E. coli
Biologically Active Protein
CASP10
>90%, by SDS-PAGE
Applications/Dilutions
Unit Definition: One unit of the recombinant caspase-10/a is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37C in a reaction solution containing 50 mM Hepes pH 7.2 50 mM NaCl 0.1% Chaps 10 mM EDTA 5% Glycerol and 10 mM DTT.
Molecular Weight: two large (18 ) and two small (11 ) subunits in a heterotetramer form.
Specific activity: > 8000 units/mg
Molecular Weight: two large (18 ) and two small (11 ) subunits in a heterotetramer form.
Specific activity: > 8000 units/mg
14.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -20 to -80C. Avoid freeze-thaw cycles.
Lyophilized powder
Lyoph
>90%, by SDS-PAGE
Reconstitute to 1 unit per l in PBS containing 15% glycerol.Following reconstitution in PBS+15% glycerol, the enzyme should be aliquoted and immediately stored at -70C. Avoid multiple freeze/thaw cycles as activity might decrease.
Alternate Names for Recombinant Human Caspase-10/FLICE2 Protein
- Apoptotic protease Mch-4
- CASP10
- CASP-10
- caspase 10, apoptosis-related cysteine peptidase
- caspase 10, apoptosis-related cysteine protease
- Caspase10
- Caspase-10
- EC 3.4.22.63
- FADD-like ICE2
- FAS-associated death domain protein interleukin-1B-converting enzyme 2
- FLICE2
- ICE-like apoptotic protease 4
- interleukin-1B-converting enzyme 2
- Mch4
- MCH4ALPS2
Background
Caspase-10/a (also known as Mch4) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-10 also exists in cells as an inactive proenzyme. During apoptosis procaspase-10 is processed at aspartate residues to form active caspase-10 which consists of two large (18 kDa) and two small (11 kDa) subunits in a heterotetramer form. The recombinant active human caspase-10 was expressed in E. coli. The active caspase-10 is routinely tested at for its ability to enzymatically cleave these two substrates Ac-IETD-pNA (Cat. #1063-200) or Ac-IETD-AFC (1062-200).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.