Recombinant Human BPNT1 Protein Summary
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-308 of Human BPNT1
Source: E.coli
Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASS NTVLMRLVAS AYSIAQKAGM IVRRVIAEGD LGIVEKTCAT DLQTKADRLA QMSICSSLAR KFPKLTIIGE EDLPSEEVDQ ELIEDSQWEE ILKQPCPSQY SAIKEEDLVV WVDPLDGTKE YTEGLLDNVT VLIGIAYEGK AIAGVINQPY YNYEAGPDAV LGRTIWGVLG LGAFGFQLKE VPAGKHIITT TRSHSNKLVT DCVAAMNPDA VLRVGGAGNK IIQLIEGKAS AYVFASPGCK KWDTCAPEVI LHAVGGKLTD IHGNVLQYHK DVKHMNSAGV LATLRNYDYY ASRVPESIKN ALVP
E. coli
Recombinant Protein
BPNT1
>90%, by SDS-PAGE
Applications/Dilutions
37.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 5mM DTT, 0.1M NaCl
No Preservative
0.5 mg/ml
>90%, by SDS-PAGE
Alternate Names for Recombinant Human BPNT1 Protein
- 3(2), 5-bisphosphate nucleotidase 1,3(2)5-bisphosphate nucleotidase 1
- Bisphosphate 3-nucleotidase 1
- BPntase
- EC 3.1.3
- EC 3.1.3.7
- PAP-inositol-1,4-phosphatase
- PIP
Background
BPNT1, also known as BPntase, PAP phosphatase or PIP (PAP-inositol-1,4-phosphatase), is a member of the magnesium-dependent, lithium-sensitive phosphomono-esterase superfamily. Using magnesium as a cofactor, BPNT1 catalyzes the conversion of PAPS (adenosine 3-phosphate 5 phosphosulfate) to APS (adenosine 5-phosphosulfate) and the conversion of PAP (3(2)-phosphoadenosine 5 phosphate) to AMP (adenosine 5-phosphate). Expressed ubiquitously with highest levels in brain and kidney, BPNT1 is potently inhibited by lithium, a drug used for the treatment of manic depression and bipolar affective disorder, suggesting a possible role for BPNT1 in the etiology of mood disorders. Recombinant human BPNT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.
product targets : HIV Integrase inhibitors