Recombinant Human BMP-2 Protein Summary
A recombinant protein corresponding to BMP2.
Source: E. coli
Amino Acid Sequence:MQAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR
Method
Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of BMP-2 as a Reference Standard.
BMP-2 is fully biologically active when compared to standard.The ED50 as determined by its ability to induce alkaline phosphatase production by ATDC-5 cells is 0.5-1.0 ug/ml.
E. coli
Biologically Active Protein
BMP2
>95%, by HPLC.
Less than 0.1 ng/ug (IEU/ug) of Bone Morphogenetic protein-2.
Applications/Dilutions
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
Lyophilized from a concentrated sterile solution containing 10mM sodium citrate pH 3.5.
No Preservative
LYOPH
>95%, by HPLC.
Reconstitute with sterilized 20 mM Acetic acid to a final concentration of at least 0.1 mg/ml.
Notes
After reconstitution, store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Alternate Names for Recombinant Human BMP-2 Protein
- BMP2
- BMP-2
- BMP-2A
- BMP2ABone morphogenetic protein 2A
- bone morphogenetic protein 2
Background
BMP2 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone morphogenic protein induces bone formation. BMP2 is a candidate gene for the autosomal dominant disease of fibrodysplasia (myositis) ossificans progressiva.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.
product targets :　FGFR inhibitors