s12888-016-0742-8

Recombinant Human BCAT2 Protein Summary

    Description
    A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 28-392 of Human BCAT2

    Source: E.coli

    Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMASSSF KAADLQLEMT QKPHKKPGPG EPLVFGKTFT DHMLMVEWND KGWGQPRIQP FQNLTLHPAS SSLHYSLQLF EGMKAFKGKD QQVRLFRPWL NMDRMLRSAM RLCLPSFDKL ELLECIRRLI EVDKDWVPDA AGTSLYVRPV LIGNEPSLGV SQPTRALLFV ILCPVGAYFP GGSVTPVSLL ADPAFIRAWV GGVGNYKLGG NYGPTVLVQQ EALKRGCEQV LWLYGPDHQL TEVGTMNIFV YWTHEDGVLE LVTPPLNGVI LPGVVRQSLL DMAQTWGEFR VVERTITMKQ LLRALEEGRV REVFGSGTAC QVCPVHRILY KDRNLHIPTM ENGPELILRF QKELKEIQYG IRAHEWMFPV

    Source
    E. coli
    Protein/Peptide Type
    Recombinant Protein
    Gene
    BCAT2
    Purity
    >90%, by SDS-PAGE

Applications/Dilutions

    Theoretical MW
    43.9 kDa.
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

    Storage
    Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
    Buffer
    20mM Tris-HCl buffer (pH 7.5) containing 10% glycerol, 0.2M NaCl, 1mM DTT
    Preservative
    No Preservative
    Concentration
    0.5 mg/ml
    Purity
    >90%, by SDS-PAGE

Alternate Names for Recombinant Human BCAT2 Protein

      BCAM
      BCAT(m)
      BCAT2
      BCATM
      BCT2
      BCT2EC 2.6.1.42
      branched chain amino-acid transaminase 2, mitochondrial
      branched chain aminotransferase 2, mitochondrial
      branched-chain-amino-acid aminotransferase, mitochondrial
      Eca40
      Placental protein 18
      PP18

Background

BCAT2 belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. This protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. It may also function as a transporter of branched chain alpha-keto acids. Recombinant human BCAT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

product targets : TGF-beta_Smad inhibitors

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