Recombinant Human ADP-Sugar Pyrophosphatase/NUDT5 Protein Summary
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-219 of Human ADP-Sugar Pyrophosphatase/NUDT5
Source: E.coli
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MESQEPTESS QNGKQYIISE ELISEGKWVK LEKTTYMDPT GKTRTWESVK RTTRKEQTAD GVAVIPVLQR TLHYECIVLV KQFRPPMGGY CIEFPAGLID DGETPEAAAL RELEEETGYK GDIAECSPAV CMDPGLSNCT IHIVTVTING DDAENARPKP KPGDGEFVEV ISLPKNDLLQ RLDALVAEEH LTVDARVYSY ALALKHANAK PFEVPFLKF
E. coli
Recombinant Protein
NUDT5
>85%, by SDS-PAGE
Applications/Dilutions
26.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl
No Preservative
1 mg/ml
>85%, by SDS-PAGE
Alternate Names for Recombinant Human ADP-Sugar Pyrophosphatase/NUDT5 Protein
- EC 3.6.1.-
- EC 3.6.1.13
- hYSAH1
- nucleoside diphosphate linked moiety X-type motif 5
- Nucleoside diphosphate-linked moiety X motif 5
- nudix (nucleoside diphosphate linked moiety X)-type motif 5
- Nudix motif 5
- NUDT5
- YSA1
- YSA1H
- YSA1HADP-sugar pyrophosphatase
Background
NUDT5 is a member of the nudix hydrolase family which eliminate toxic nucleotide derivatives from the cell. NUDT5 hydrolyzes ADP-ribose and ADP-mannose in the presence of magnesium, and also hydrolyzes other nucleotide sugars with low activity such as ADP-glucose and diadenosine diphosphate. As a nudix hydrolase, NUDT5 contains a central nudix motif and functions to eliminate toxic nucleotide metabolites from the cell while maintaining the levels of signaling nucleotides. NUDT5 is widely expressed but is most abundant in liver as a homodimer. Recombinant human NUDT5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.
product targets :　Deubiquitinase inhibitors