j.jgr.2015.12.001

Recombinant Human 4-1BB/TNFRSF9/CD137 Protein Summary

    Description
    Recombinant bioactive protein containing 167 amino acids for Human 4-1BB/TNFRSF9/CD137

    Source:E. coli
    Amino Acid Sequence:(Q07011 )

    Preparation
    Method
    >98% SDS-PAGE
    Details of Functionality
    Determined by its inhibitory effect of the 4-1BBL mediated stimulation of IL-8 production by human PBMC. About 90% of inhibition was seen using a concentration of 1 ug for both 4-1BBL and 4-1BBR.
    Source
    E. coli
    Protein/Peptide Type
    Biologically Active Protein
    Gene
    TNFRSF9
    Purity
    >95%, by SDS-PAGE
    Endotoxin Note
    <0.1 ng/ug

Applications/Dilutions

    Theoretical MW
    17.8 kDa.
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

    Storage
    Store at -20 to -80C. Avoid freeze-thaw cycles.
    Buffer
    Lyophilized with no additives
    Concentration
    Lyoph
    Purity
    >95%, by SDS-PAGE
    Reconstitution Instructions
    Reconstitute in H2O to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use.

Alternate Names for Recombinant Human 4-1BB/TNFRSF9/CD137 Protein

      4-1BB ligand receptor
      41BB
      4-1BB
      CD137 antigen
      CD137
      CD137MGC2172
      CDw137
      FLJ43501
      ILA
      ILAhomolog of mouse 4-1BB
      induced by lymphocyte activation (ILA)
      interleukin-activated receptor, homolog of mouse Ly63
      receptor protein 4-1BB
      T cell antigen ILA
      T-cell antigen 4-1BB homolog
      T-cell antigen ILA
      TNFRSF9
      tumor necrosis factor receptor superfamily member 9
      tumor necrosis factor receptor superfamily, member 9

Background

Human 4-1BB Receptor (4-1BBR) is an inhibitor of 4-1BBL and can block 4-1BBL bioactivity. Recombinant human 4-1BBR is a 17.8 kDa protein containing 167 amino acid residues of extraCellular domain of 4-1BBR without signal peptide (Gene accession # NP_001552).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

(-)-Blebbistatin

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