Rat TNF-alpha ELISA Kit (Colorimetric) Summary
39.1 – 2,500 pg/mL
11.0 pg/mL
Sandwich ELISA
10%
5.0%
109%
50 uL
ELISA Kit (Colorimetric)
TNF
Applications/Dilutions
The rat TNF-alpha ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of rat TNF-alpha.
-
ELISA1 publication
NBP1-92681 in the following applications:
Reactivity Notes
Rat
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
Kit Components
- Biotin-Conjugate anti-rat TNF-alpha polyclonal antibody
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to rat TNF-alpha
- Adhesive Films
- Stop Solution (1M Phosphoric acid)
- Rat TNF-alpha Standard lyophilized, 5 ng/mL upon reconstitution
- Sample Diluent
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
- Streptavidin-HRP
- Substrate Solution (tetramethyl-benzidine)
Alternate Names for Rat TNF-alpha ELISA Kit (Colorimetric)
- APC1 protein
- Cachectin
- Cachetin
- DIF
- TNF
- TNF, monocyte-derived
- tnfa
- tnf-a
- TNFalpha
- TNF-alpha
- TNF-alphacachectin
- TNFATNF, macrophage-derived
- TNFSF1A
- TNFSF2
- TNFSF2TNF superfamily, member 2
- tumor necrosis factor (TNF superfamily, member 2)
- tumor necrosis factor alpha
- Tumor necrosis factor ligand superfamily member 2
- tumor necrosis factor
- tumor necrosis factor-alpha
Background
TNF-alpha release is mainly triggered by viral infections, endotoxins, lipopolysaccharides or other bacterial components, by tissue injury, DNA-damage and by IL-1, PDFG and TNF-alpha itself. It is primarily expressed in macrophages, but also in monocytes, neutrophils, NK-cells, mast-cells, endothelial cells and activated lymphocytes. TNF-alpha expression in endothelial cells and fibroblasts can be induced by IL-17. The expression of other cytokines, chemokines, reactive oxygen intermediates, nitric oxide and prostaglandins is stimulated by TNF-alpha. Multiple biological functions of TNF-alpha comprise cellular proliferation and differentiation, tumorigenesis, apoptotic or necrotic cell death (including certain tumor cell lines), immunoregulatory activities, lipid metabolism, coagulation and endothelial function. It promotes local or systemic inflammation (TNF-alpha is a potent pyrogen) and stimulates the acute phase response. Very high expressions of TNF-alpha after infection can lead to septic shock (TNF-alpha is highly cytotoxic.), whereas sustained low levels induce cachexia and inflammation. Dysregulation of TNF-alpha is involved in many diseases.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.