bmj.39231.450845.59

Rat TGF-beta 1 ELISA Kit Summary

    Standard Curve Range
    31.3 – 2,000 pg/mL
    Sensitivity
    8.0 pg/mL
    Sample Volume
    100 uL (pretreated)
    Kit Type
    ELISA Kit
    Gene
    TGFB1

Applications/Dilutions

    Application Notes
    The rat TGF-beta1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of rat TGF-beta1

Reactivity Notes

Rat

Packaging, Storage & Formulations

    Storage
    Storage of components varies. See protocol for specific instructions.

Kit Components

Components
      Streptavidin-HRP
      Substrat Solution (tetramethyl-benzidine)
      Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
      Rat TGF-beta1 Standard lyophilized, 4000 pg/ml upon reconstitution
      Stop Solution (1M Phosphoric acid)
      Adhesive Films
      Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to rat TGF-beta1
      Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
      Biotin-Conjugate anti-rat TGF-beta1 polyclonal antibody

Alternate Names for Rat TGF-beta 1 ELISA Kit

      CEDLAP
      DPD1
      latency-associated peptide
      TGFB
      TGFB1
      TGF-beta 1 protein
      TGFbeta 1
      TGF-beta 1
      TGFbeta
      TGF-beta-1
      transforming growth factor beta-1
      transforming growth factor, beta 1

Background

TGF-beta plays a critical role in cellular growth, development, differentiation, proliferation, extracellular matrix (ECM) synthesis and degradation, control of mesenchymal-epithelial interactions during embryogenesis, immune modulation, apoptosis, cell cycle progression, angiogenesis, adhesion and migration and leukocyte chemotaxis. It has both tumor suppressive and tumor promoting activities and is highly regulated at all levels. TGF-beta1 is the key mediator in the pathophysiology of tissue repair and human fibrogenesis: balance between production and degradation of type I collagen, and fibrosis and scarring in organ and tissue. TGF-beta1 promotes Treg cell differentiation resulting in IL-10/TGF-beta1 production and Th1 cell and Th2 cell suppression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Volasertib

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