Rat IL-4 ELISA Kit (Colorimetric) Summary
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a rat IL-4 positive serum. There was no crossreactivity detected.
1.6 – 100 pg/mL
0.2 pg/mL
Sandwich ELISA
10%
5.0%
50 uL
ELISA Kit (Colorimetric)
IL4
Applications/Dilutions
This kit is an enzyme-linked immunosorbent assay for quantitative detection.
Reactivity Notes
Rat
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
Kit Components
- 1 vial (55 ul) Biotin-Conjugate anti-rat IL-4 monoclonal antibody
- 1 vial rat IL-4 Standard protein lyophilized, 2 ng/ml upon reconstitution
- 1 vial (11 ul) Streptavidin-HRP
- 1 vial (1.1 ml) monoclonal Coating Antibody to rat IL-4 (100 ug/ml)
- 2 vials (50 ml) Sample Diluent
Alternate Names for Rat IL-4 ELISA Kit (Colorimetric)
- B cell growth factor 1
- BCDF
- B-cell stimulatory factor 1
- BCGF1
- BCGF-1
- binetrakin
- BSF1
- BSF-1
- IL4
- IL-4
- IL-4B_cell stimulatory factor 1
- interleukin 4
- interleukin-4
- Lymphocyte stimulatory factor 1
- MGC79402
- pitrakinra
Background
IL-4 mediates its function by binding to receptors expressed on target cells. The IL-4 receptors exhibit an affinity of approximately 10-10 M. Receptors exist on freshly prepared B and T lymphocytes and macrophages, as well as on various cell lines including lymphoid cells, mast cell lines, a variety of other hematopoietic cell lines, fibroblasts and stromal cell lines. On T and B cells, receptors are present in low numbers (appr. 400), which are reported to be upregulated by IL-2 and IL-4. IL-4 is produced by a particular subset of T helper cells, the TH2 cells.These cells tend to make a specific set of lymphokines including IL-4, IL-5, IL-6, IL-10, IL-13 and GM-CSF and fail to produce IL-2, IFN-gamma, and lymphotoxin (TNF-beta). Apart from T cells, it has been shown that mast cells can produce IL-4. IL-4 exerts numerous effects on various hematopoietic cell types. On B cells, IL-4 promotes immunological class switching to IgE and IgG1 isotypes and upregulates MHC class II and CD23 expression. It can promote survival, growth, and differentiation of both T and B lymphocytes, mast cells, and endothelial cells. In addition, IL-4 can inhibit the production of TNF, IL-1, and IL-6 by macrophages.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.