Rat IL-1 beta/IL-1F2 ELISA Kit (Colorimetric) Summary
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a rat IL-1 beta positive serum.
31.3 – 2,000 pg/mL
4.0 pg/mL
Sandwich ELISA
10%
10%
The unspiked serum was used as blank in these experiments. The overall mean recovery was 88%.
50 uL
ELISA Kit (Colorimetric)
IL1B
Applications/Dilutions
This kit is an enzyme-linked immunosorbent assay for quantitative detection.
Reactivity Notes
Rat
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
Kit Components
- Stop Solution (1M Phosphoric acid)
- Sample Diluent
- Rat IL-1beta Standard lyophilized, 4 ng/ml upon reconstitution
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
- Streptavidin-HRP
- Substrate Solution (tetramethyl-benzidine)
- Biotin-Conjugate anti-rat IL-1beta polyclonal antibody
- Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to rat IL-1beta
- Adhesive Films
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Alternate Names for Rat IL-1 beta/IL-1F2 ELISA Kit (Colorimetric)
- catabolin
- IL1 beta
- IL-1 beta
- IL-1
- IL1B
- IL-1b
- IL1-BETA
- IL-1F2
- IL1F2IL-1 beta
- interleukin 1, beta
- interleukin-1 beta
- preinterleukin 1 beta
- pro-interleukin-1-beta
Background
IL-1beta is a pro-inflamatory cytokine and is expressed by many cells including macrophage, NK cells, monocytes, and neutrophils. It belongs to the IL-1 family cluster. Associated activities with IL-1 are the induction of Prostaglandin E2 by synovial cells and release of collagenase with resulting destruction of cartilage and bone resorption. IL-1, has multiple immunological functions including enhancement of IL-2 production by T cells and activation of B-cells (BAF) and thymocytes.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.