Rat IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric) Summary
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a rat IL-1 alpha positive serum. There was no crossreactivity detected
15.6 – 1,000 pg/mL
4.0 pg/mL
Sandwich ELISA
10%
5.0%
50 uL
ELISA Kit (Colorimetric)
IL1A
Applications/Dilutions
The rat IL-1alpha ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of rat IL-1alpha
Reactivity Notes
Rat
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
Kit Components
- Biotin-Conjugate anti-rat IL-1alpha polyclonal antibody
- Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to rat IL-1alpha
- Adhesive Films
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Stop Solution (1M Phosphoric acid)
- Rat IL-1alpha Standard lyophilized, 2 ng/ml upon reconstitution
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
- Streptavidin-HRP
- Substrate Solution (tetramethyl-benzidine)
Alternate Names for Rat IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric)
- Hematopoietin-1
- IL1 alpha
- IL-1 alpha
- IL1
- IL1A
- IL-1A
- IL1-ALPHA
- IL-1F1
- IL1F1hematopoietin-1
- interleukin 1, alpha
- interleukin-1 alpha
- preinterleukin 1 alpha
- pro-interleukin-1-alpha
Background
The interleukin-1 species represent an important family of biologically active mono nuclear cell-derived proteins which are involved in inflammatory reactions and in immune responses. Two distinct IL-1 species, IL-1alpha and IL-1beta, have been identified. They share similarities such as the same molecular weight, similar biological effects and the same receptors on target cells. IL-1 proteins are produced by macrophages, monocytes and various other cell types such as adult T cell leukemias, fibroblasts, epithelial or endothelial cells, neutrophils and astrocytes. Their biological properties include pyrogenicity, bone resorption, presentation of antigen to T cells and stimulation of B and T lymphocyte proliferation.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.