Rat IL-10 ELISA Kit (Colorimetric) Summary
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a rat IL-10 positive serum. There was no crossreactivity detected.
15.6 – 1,000 pg/mL
1.5 pg/mL
Sandwich ELISA
10%
5.0%
50 uL
ELISA Kit (Colorimetric)
IL10
Applications/Dilutions
This kit is an enzyme-linked immunosorbent assay for quantitative detection.
Reactivity Notes
Rat
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
Kit Components
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Adhesive Films
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to rat IL-10
- Biotin-Conjugate anti-rat IL-10 monoclonal antibody
- Substrate Solution (tetramethyl-benzidine)
- Streptavidin-HRP
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
- Rat IL-10 Standard lyophilized, 2 ng/ml upon reconstitution
- Sample Diluent
- Stop Solution (1M Phosphoric acid)
Alternate Names for Rat IL-10 ELISA Kit (Colorimetric)
- CSIF
- CSIFMGC126450
- Cytokine synthesis inhibitory factor
- IL10
- IL-10
- IL10A
- IL-10MGC126451
- interleukin 10
- interleukin-10
- TGIF
Background
Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages, T-cells and NK cells. In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes. The murine IL-10 exhibits strong DNA and aminoacid sequence homology to the human IL-10 and an open reading frame in the Epstein- Barr virus genome, BCRF1 which shares many of the cellular cytokines biological activities and may therefore play a role in the host-virus interaction.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.