eLife.08595

RUNX2/CBFA1 Antibody Summary

    Immunogen
    RUNX2 (NP_004339, 251 a.a. – 350 a.a.) partial recombinant protein with GST tag. NPRPSLNSAPSPFNPQGQSQITDPRQAQSSPPWSYDQSYPSYLSQMTSPSIHSTTPLSSTRGTGLPAITDVPRRISDDDTATSDFCLWPSTLSKKSQAGA
    Specificity
    RUNX2 – runt-related transcription factor 2
    Isotype
    IgG
    Clonality
    Polyclonal
    Host
    Mouse
    Gene
    RUNX2
    Purity
    Unpurified
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Applications/Dilutions

    Dilutions
        Western Blot
        ELISA
    Application Notes
    The quality control of this antibody is limited to WB on the immunizing protein. It has been used for ELISA. Abnovas recommended working dilutions for western analysis are as follows: 1:500 dilution for ascites 1:1000 for purified Ig 1:500

Packaging, Storage & Formulations

    Storage
    Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
    Buffer
    Whole antisera with 50% Glycerol
    Preservative
    No Preservative
    Purity
    Unpurified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RUNX2/CBFA1 Antibody

      Acute myeloid leukemia 3 protein
      AML3CCD
      CBFA1
      CBFA1MGC120022
      Core-binding factor subunit alpha-1
      MGC120023
      PEA2-alpha A
      PEBP2A
      PEBP2A1CCD1
      PEBP2aA1
      PEBP2-alpha A
      runt domain, alpha subunit 1
      runt-related transcription factor 2
      RUNX2
      SL3/AKV core-binding factor alpha A subunit
      SL3-3 enhancer factor 1 alpha A subunit

Background

This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

JWH-134

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