RNMT [p Tyr1235] Antibody (NL8) Summary
KLH-conjugated, synthetic peptide in which pYpY corresponds to phosphorylated amino acids 1234 and 1234 of human Met/HGFR.
p Tyr1235
Recognizes phosphorylated Met, Mr 140 kDa. Cross-reacts with phosphorylated EGFR.
Monoclonal
Rabbit
RNMT
Tissue culture supernatant
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovators Reward
Applications/Dilutions
- Western Blot 1:100-1:2000
140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reactivity Notes
Human
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
No buffer
0.05% Sodium Azide
Tissue culture supernatant
Notes
Relevant Entrez Gene Numbers: NP_000236.2
Alternate Names for RNMT [p Tyr1235] Antibody (NL8)
- DKFZp686H1252
- EC 2.1.1.56
- hcm1p
- hCMT1
- hCMT1c
- hMet
- KIAA0398
- mRNA (guanine-7-)methyltransferase
- mRNA (guanine-N(7)-)-methyltransferase
- mRNA cap guanine-N7 methyltransferase
- mRNA cap methyltransferase
- RG7MT1MET
- RNA (guanine-7-) methyltransferase
Background
The proto-oncogene Met (c-Met, HGF/SF Receptor, HGFR) is a receptor tyrosine kinase that is activated upon Hepatocyte Growth Factor (HGF) binding. This stimulates the autophosphorylation on Tyr1234/Tyr1235 which leads to pleiotropic downstream signaling events in epithelial cells that stimulate multiple cellular processes including cell proliferation, growth, differentiation, cell migration/ invasion, and tumorigenesis. These effects are mediated by SH2 domain-containing proteins that bind to the C-terminal tail of c-Met. Chronic stimulation of Met on cancer cells is thought to play a role in metastasis and dysregulation of c-Met activity is directly associated with oncogenesis, making it an important cancer target.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.