17453674.2016.1203477

Prothrombin ELISA Kit (Colorimetric) Summary

    Description
    Prothrombin Screen ELISA Kit is an indirect solid phase ELISA for the simultaneous quantitative measurement of IgG IgM and IgA class autoantibodies against Prothrombin in human serum or plasma.
    Standard Curve Range
    6.3 to 100 U/mL
    Sensitivity
    1.0 U/mL
    Assay Type
    Sandwich ELISA
    Inter-Assay
    CV% < 2.0-9.4%
    Intra-Assay
    CV% < 2.2-6.0%
    Sample Volume
    10 uL
    Kit Type
    ELISA Kit (Colorimetric)
    Gene
    F2

Applications/Dilutions

    Application Notes
    Useful for quantification.

Packaging, Storage & Formulations

    Storage
    Store at 4°C.

Kit Components

Components
      Enzyme conjugate containing anti-human IgG, IgA and IgM antibodies, HRP labelled;PBS, BSA, detergent, preservative ProClin 300 0.05%, light red. Ready to use.
      Divisible microplate consisting of 12 modules of 8 wells each. Ready to use.
      Calibrators A-F (0, 6.3, 12.5, 25, 50, 100 U/mL), containing serum/buffer matrix (PBS,BSA, detergent, NaN3 0.09%), yellow. Ready to use.
      Control positive (1) and negative (2), containing prothrombin antibodies in aserum/buffer matrix (PBS, BSA, detergent, NaN3 0.09%), yellow, Ready to use. Theconcentration is specified on the certificate of analysis.
      Wash solution, containing Tris, detergent, preservative NaN3 0.09%; 50x concentrated.
      TMB substrate, containing 3,3’,5,5’-Tetramethylbenzidin, colorless. Ready to use.
      Sample buffer P, containing PBS, BSA, detergent, preservative NaN3 0.09%, yellow,concentrate (5x).
      Stop solution, contains acid. Ready to use.

Notes

Quality control test: Standard curve.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Prothrombin ELISA Kit (Colorimetric)

      coagulation factor II (thrombin)
      Coagulation factor II
      EC 3.4.21
      EC 3.4.21.5
      prothrombin B-chain
      prothrombin
      PT
      serine protease

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

product targets : Procollagen C Proteinase inhibitors

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