Primate P-Selectin/CD62P ELISA Kit (Colorimetric) Summary
0.8 – 50 U/mL
1.3 U/mL
Sandwich ELISA
10%
10%
10 uL
ELISA Kit (Colorimetric)
SELP
Applications/Dilutions
The monkey sP-selectin ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of monkey sP-selectin.
Reactivity Notes
Monkey
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
Kit Components
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to monkey sP-selectin
- Adhesive Films
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Monkey sP-selectin Standard lyophilized, 100.0 U/ml upon reconstitution
- HRP-Conjugate anti-monkey sP-selectin monoclonal antibody
- Stop Solution (1M Phosphoric acid)
- Sample Diluent
- Substrate Solution (tetramethyl-benzidine)
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Alternate Names for Primate P-Selectin/CD62P ELISA Kit (Colorimetric)
- CD62P antigen
- CD62P
- FLJ45155
- GMP140
- GRMP
- PADGEM
- PADGEMantigen CD62)
- PSEL
- P-Selectin
- selectin P (granule membrane protein 140kDa, antigen CD62)
- SELP
Background
P-selectin belongs to the selectin family of adhesion molecules. P-selectin acts as a receptor that supports binding of leukocytes to activated platelets and endothelium. P-selectin is a 140 kDa protein that is highly glycosylated. The cDNA derived amino acid sequence predicts a molecule with a series of cysteine-rich domains. P-selectin is located in membranes of a granules in unstimulated platelets and redistributed to the cell surface upon platelet activation. P-selectin is also present in endothelial cells in membranes of Weibel-Palade bodies and megakaryocytes. The physiologic role of P-selectin might be the mediation of initial leukocyte adhesion to activated endothelium during acute inflammation. It may work in concert with E-selectin to direct early, regionally specific adherence of neutrophils and monocytes at sites of acute inflammation. A soluble form of P-selectin found in serum and plasma has been described which might represent a proteolytic fragment or more likely a soluble splice variant lacking the transmembrane domain. Excessive accumulation of neutrophils on the endothelial surface accompanied by high exposure of P-selectin has been implicated in a number of inflammatory disorders, including adult respiratory distress syndrome, acute lung injury, ischemia-reperfusion injury, Gram-negative septic shock, thrombotic diseases and rheumatoid arthritis. Malignant cells were shown to express receptors for P-selectin suggesting an important role for P-selectin in tumor formation and metastasis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.