Primate CXCL8/IL-8 ELISA Kit (Colorimetric) Summary
19.5 – 1,250 U/mL
3.0 U/mL
Sandwich ELISA
8.7%
6.3%
50 uL
ELISA Kit (Colorimetric)
IL8
Applications/Dilutions
The monkey IL-8 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of monkey IL-8
Reactivity Notes
Monkey
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
Kit Components
- Substrate Solution (tetramethyl-benzidine)
- Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
- Sample Diluent (Use when an external predilution of the samples is needed)
- Stop Solution (1M Phosphoric acid)
- Aluminium pouch(es) with a monkey IL-8 Standard curve (coloured)
- Aluminium pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to monkey IL-8, Biotin-Conjugate (anti-IL-8 polyclonal antibody), Streptavidin-HRP and Sample Diluent, lyophilized
- Adhesive Plate Covers
Alternate Names for Primate CXCL8/IL-8 ELISA Kit (Colorimetric)
- 3-10C
- AMCF-I
- C-X-C motif chemokine 8
- CXCL8
- CXCL8SCYB8
- Emoctakin
- GCP1
- GCP-1TSG-1
- IL8
- IL-8
- interleukin 8
- K60
- LAI
- LECT
- MDNCF
- MDNCFb-ENAP
- member 8
- MONAPGCP1
- NAP1
- NAP-1NAP1
- NCF
- Neutrophil-activating protein 1
- Protein 3-10C
- T cell chemotactic factor
- T-cell chemotactic factor
- TCF
- TSG1
Background
Interleukin-8 selectively stimulates the ability of neutrophils and T-lymphocytes to invade injured or inflamed tissue. IL-8 also known as CXCL8 that is purified from peripheral blood monocytes is a 10 kDa protein showing at least 4 variant forms (77, 72, 70, and 69 amino acids, respectively) which differ in the length of the N-terminal and readily form dimers in solution. Two cystin-bridges are essential for the biological activity of IL-8. Exogenous stimuli like LPS, but also IL-1, TNF-alpha and TNF-beta induce the secretion of IL-8 in a variety of different cell types including monocytes, endothelial and epithelial cells, peripheral blood mononuclear cells, dermal fibroblasts, keratinocytes, neutrophils, hepatocytes, synovial cells, and T-lymphocytes. The activities of IL-8 are not species-specific. Human IL-8 is also active in rodent and rabbit cells and differs from all other cytokines in its ability to specifically activate neutrophil granulocytes. This chemokine activates multiple intracellular signaling pathways downstream of two cell-surface, G protein-coupled receptors (CXCR1 and CXCR2). The property of IL-8 to stimulate movement of neutrophils across endothelial monolayers in vitro supports the concept of a central role for this molecule in the accumulation of neutrophils at inflammatory lesions in vivo.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.