PI 3-Kinase p85 alpha Antibody (U13) Summary
Baculovirus expressed (recombinant) Bovine p85 alpha subunit of PI 3 Kinase p85 alpha.
Cytoplasmic
Recognizes the SH3 domain of the p85 alpha subunit of bovine PI 3 kinase.
IgG1
Monoclonal
Mouse
PIK3R1
Tissue culture supernatant
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Applications/Dilutions
- Western Blot 1:200
- Immunoprecipitation 1:10-1:500
Reactivity Notes
Cross-reacts with Human, Monkey, Mouse, Bovine and Rat. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.2M Tris/HCl (pH 7.4) and 5 – 10% fetal Bovine serum (FBS)
0.1% Sodium Azide
Tissue culture supernatant
Alternate Names for PI 3-Kinase p85 alpha Antibody (U13)
- GRB1
- GRB1PI3-kinase subunit p85-alpha
- p85
- p85-ALPHA
- Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha
- phosphatidylinositol 3-kinase regulatory subunit alpha
- phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
- phosphatidylinositol 3-kinase, regulatory, 1
- phosphatidylinositol 3-kinase-associated p-85 alpha
- phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
- PI 3Kinase p85 alpha
- PI 3-Kinase p85 alpha
- PI3K regulatory subunit alpha
- PI3-kinase regulatory subunit alpha
- PIK3R1
- PtdIns-3-kinase regulatory subunit alpha
- PtdIns-3-kinase regulatory subunit p85-alpha
Background
The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
product targets :　Inflammation/Immunology inhibitors