s12889-015-2215-2

NCF4 Antibody (3C10) Summary

    Immunogen
    NCF4 (AAH02798, 1 a.a. – 100 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAVAQQLRAESDFEQLPDDVAISANIADIEEKRGFTSHFVFVIEVKTKGGSKYLIYRRYRQFHALQSKLEERFGPDSKSSALACTLPTLPAKVYVGVKQE
    Localization
    Cytoplasmic
    Specificity
    NCF4 – neutrophil cytosolic factor 4, 40kDa
    Isotype
    IgG2a Kappa
    Clonality
    Monoclonal
    Host
    Mouse
    Gene
    NCF4
    Purity
    IgG purified
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Applications/Dilutions

    Dilutions
        ELISA
    Application Notes
    Antibody reactivity against recombinant protein on ELISA. GST alone used as a negative control.

Packaging, Storage & Formulations

    Storage
    Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
    Buffer
    PBS (pH 7.4)
    Preservative
    No Preservative
    Purity
    IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NCF4 Antibody (3C10)

      MGC3810
      NCF
      NCF-4
      neutrophil cytosol factor 4
      neutrophil cytosolic factor 4 (40kD)
      neutrophil cytosolic factor 4, 40kDa
      Neutrophil NADPH oxidase factor 4
      p40phox
      p40-phox
      SH3 and PX domain-containing protein 4
      SH3PXD4P40PHOX

Background

The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

ONX-0916

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