Myosin Phosphatase [p Thr853] Antibody Summary
Synthetic peptide corresponding to aa845-855, C-EKRRS[pT]GVSFW of chicken MYPT1 with an N-terminal cysteine added for conjugation purposes. Phosphorylation site corresponds to Thr853 in human MYPT1.
p Thr853
Recognizes recombinant human truncated MYPT1 phosphorylated on Thr853, MW approx 80kD.
IgG
Polyclonal
Rabbit
PPP1R12A
Protein A purified
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Applications/Dilutions
- Western Blot 0.5-2 ug/ml
Western Blot: Detects MYPT1 in C2C12 lysates treated with pervanadate.
Reactivity Notes
Chicken (Thr850), Mouse (Thr852) and Rat (Thr855)
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.1M Tris-Glycine (pH 7.4), 0.15M NaCl and 30% Glycerol
0.05% Sodium Azide
1 mg/ml
Protein A purified
Alternate Names for Myosin Phosphatase [p Thr853] Antibody
- MBSmyosin phosphatase, target subunit 1
- MGC133042
- Myosin phosphatase target subunit 1
- Myosin phosphatase-targeting subunit 1
- MYPT1protein phosphatase 1 regulatory subunit 12A
- protein phosphatase 1, regulatory (inhibitor) subunit 12A
- Protein phosphatase myosin-binding subunit
Background
Myosin phosphatase target subunit 1 (MYPT1), which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho, as previously shown (6). To understand the role of MYPT1 in the regulation of the cytoskeleton in human diseases, a 4855-bp cDNA was cloned for human MYPT1 using the rat MYPT1 cDNA as probe. Sequencing analysis has revealed that human MYPT1 contains 1030 amino acid residues with a calculated molecular mass of approximately 115kD. Fluorescence in situ hybridization analysis placed the human MYPT1 gene on chromosome 12q15-q21.2. Radiation hybrid mapping has shown that the human MYPT1 gene is located very near the highly polymorphic marker CHLC.GATA65A12, which lies between D12S350 and D12S106.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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