Mouse TNF-alpha ELISA Kit (Colorimetric) Summary
Applications/Dilutions
Reactivity Notes
Packaging, Storage & Formulations
Kit Components
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
- Substrate Solution (tetramethyl-benzidine)
- Streptavidin-HRP
- Stop Solution (1M Phosphoric acid)
- Sample Diluent
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to mouse TNF-alpha
- Adhesive Films
- Mouse TNF-alpha Standard lyophilized, 2 ng/mL upon reconstitution
- Calibrator Diluent
- Biotin-Conjugate anti-mouse TNF-alpha polyclonal antibody
Alternate Names for Mouse TNF-alpha ELISA Kit (Colorimetric)
- APC1 protein
- Cachectin
- Cachetin
- DIF
- TNF
- TNF, monocyte-derived
- tnfa
- tnf-a
- TNFalpha
- TNF-alpha
- TNF-alphacachectin
- TNFATNF, macrophage-derived
- TNFSF1A
- TNFSF2
- TNFSF2TNF superfamily, member 2
- tumor necrosis factor (TNF superfamily, member 2)
- tumor necrosis factor alpha
- Tumor necrosis factor ligand superfamily member 2
- tumor necrosis factor
- tumor necrosis factor-alpha
Background
Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.
TNF-alpha release is mainly triggered by viral infections, endotoxins, lipopolysaccharides or other bacterial components, by tissue injury, DNA-damage and by IL-1, PDGF and TNF-alpha itself. It is primarily expressed in macrophages, but also in monocytes, neutrophils, NK-cells, mast-cells, endothelial cells and activated lymphocytes. TNF-alpha expression in endothelial cells and fibroblasts can be induced by IL-17. The expression of other cytokines, chemokines, reactive oxygen intermediates, nitric oxide and prostaglandins is stimulated by TNF-alpha.
Multiple biological functions of TNF-alpha comprise cellular proliferation and differentiation, tumorigenesis, apoptotic or necrotic cell death (including certain tumor cell lines), immunoregulatory activities, lipid metabolism, coagulation and endothelial function. It promotes local or systemic inflammation (TNF-alpha is a potent pyrogen) and stimulates the acute phase response. Very high expressions of TNF-alpha after infection can lead to septic shock (TNF-alpha is highly cytotoxic.), whereas sustained low levels induce cachexia and inflammation. Dysregulation of TNF-alpha is involved in many diseases.