Mouse IL-6 ELISA Kit (Colorimetric) Summary
The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a mouse IL-6 positive serum. There was no cross reactivity observed with mouse IL-17, IL-2, IFN-gamma, TFN-alpha, IL-5, IL-1alpha, GM-CSF, IL-4, IL-10.
31.3 – 2,000 pg/mL
6.5 pg/mL
Sandwich ELISA
8.9%
5.0%
Please see protocol for more specific Spike Recovery details.
50ul
ELISA Kit (Colorimetric)
IL6
Applications/Dilutions
This Mouse IL-6 ELISA contains the necessary reagents, from pre-coated plates to standards, buffers and diluents for performing quantitative enzyme-linked immunosorbent assays (ELISA). This ELISA kit is specifically engineered for accurate and precise measurement of IL-6 protein levels from samples including serum, plasma, and supernatants from cell cultures.
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
Kit Components
- * reagent contains ethyl alcohol
- Assay Buffer
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-6
- Amplification Diluent Concentrate (2x)
- Amplification Reagent I*
- Amplification Reagent II
- Adhesive Films
- Mouse IL-6 Standard lyophilized, 4 ng/mL upon reconstitution
- Biotin-Conjugate anti-mouse IL-6 monoclonal antibody
- Sample Diluent
- Stop Solution (1M Phosphoric acid)
- Streptavidin-HRP
- Substrate Solution (tetramethyl-benzidine)
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Alternate Names for Mouse IL-6 ELISA Kit (Colorimetric)
- B cell stimulatory factor-2
- B-cell differentiation factor
- BSF-2
- BSF2CTL differentiation factor
- CDF
- HGFHSFIFNB2Hybridoma growth factor
- IFN-beta-2
- IL6
- IL-6
- IL-6B-cell stimulatory factor 2
- Interferon beta-2
- interleukin 6 (interferon, beta 2)
- interleukin BSF-2
- interleukin-6
- MGI-2A
Background
Interleukin-6 (IL-6) is a multi-functional cytokine that regulates immune responses, acute phase reactions and hematopoiesis and may play a central role in host defense mechanisms. IL-6 is usually not produced constitutively by normal cells, but its expression is readily induced by a variety of cytokines,lipopolysaccharide (LPS) or viral infections. IL-6 is a pleiotropic cytokine produced by a variety of cells. It acts on a wide range of tissues, exerting growth-induction, growth-inhibition, and differentiation respectively, depending on the nature of the target cells. IL-6 is involved in: – the induction of B-cell differentiation – the induction of acute phase proteins in liver cells – growth promotion of myeloma/plasmacytoma/hybridoma cells – induction of IL-2 and IL-2 receptor expression – proliferation and differentiation of T cells – inhibition of cell growth of certain myeloid leukemic cell lines and induction of their differentiation to macrophages – enhancement of IL-3-induced multipotential colony cell formation in hematopoietic stem cells and induction of maturation of megakaryocytes as a thrombopoietic factor – induction of mesangial cell growth – induction of neural differentiation of PC 12 cells – induction of keratinocyte growth The abnormal production of IL-6 was first suggested to be related to polyclonal B-cell activation with autoantibody production in patients with cardiac myxoma. Since then, IL-6 has been suggested to be involved in the pathogenesis of a variety of diseases. Measurement of IL-6 levels in serum and other body fluids thus provides more detailed insights into various pathological situations.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.