Mouse IL-4 ELISA Kit (Colorimetric) Summary
Applications/Dilutions
Reactivity Notes
Packaging, Storage & Formulations
Kit Components
- Substrate Solution
- Streptavidin-HRP
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
- Sample Diluent
- Stop Solution (1M Phosphoric acid)
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-4
- Amplification Reagent II
- Amplification Reagent I*
- Amplification Diluent Concentrate (2x)
- Adhesive Films
- Assay Diluent
- * reagent contains ethyl alcohol
- Biotin-Conjugate anti-mouse IL-4 monoclonal antibody
- Conjugate Diluent Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Mouse IL-4 Standard lyophilized, 500 pg/ml upon reconstitution
Alternate Names for Mouse IL-4 ELISA Kit (Colorimetric)
- B cell growth factor 1
- BCDF
- B-cell stimulatory factor 1
- BCGF1
- BCGF-1
- binetrakin
- BSF1
- BSF-1
- IL4
- IL-4
- IL-4B_cell stimulatory factor 1
- interleukin 4
- interleukin-4
- Lymphocyte stimulatory factor 1
- MGC79402
- pitrakinra
Background
IL-4 mediates its function by binding to receptors expressed on target cells. The IL-4 receptors exhibit an affinity of approximately 10-10 M.
Receptors exist on freshly prepared B and T lymphocytes and macrophages, as well as on various cell lines including lymphoid cells, mast cell lines, a variety of other hematopoietic cell lines, fibroblasts and stromal cell lines. On T and B cells, receptors are present in low numbers (appr. 400), which are reported to be upregulated by IL-2 and IL-4.
IL-4 is produced by a particular subset of T helper cells, the TH2 cells. These cells tend to make a specific set of lymphokines including IL-4, IL-5, IL-6, IL-10, IL-13 and GM-CSF and fail to produce IL-2, IFN-gamma, and lymphotoxin (TNF-beta). Apart from T cells, it has been shown that mast cells can produce IL-4.
IL-4 exerts numerous effects on various hematopoietic cell types. On B cells, IL-4 promotes immunological class switching to IgE and IgG1 isotypes and upregulates MHC class II and CD23 expression. It can promote survival, growth, and differentiation of both T and B lymphocytes, mast cells, and endothelial cells. In addition, IL-4 can inhibit the production of TNF, IL-1, and IL-6 by macrophages..