Mouse IL-17/IL-17A ELISA Kit (Colorimetric) Summary
The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a mouse IL-17 positive serum. There was no crossreactivity detected
7.8 – 500 pg/mL
1.6 pg/mL
Sandwich ELISA
8.50%
9.10%
The overall mean recovery was 80%. Please see protocol for more specific Spike Recovery details.
50 uL
ELISA Kit (Colorimetric)
IL17A
Applications/Dilutions
The mouse IL-17 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-17.
Reactivity Notes
Mouse
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
Kit Components
- Mouse IL-17 Standard lyophilized, 1 ng/ml upon reconstitution
- Biotin-Conjugate anti-mouse IL-17 monoclonal antibody
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-17
- Adhesive Films
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
- Substrate Solution (tetramethyl-benzidine)
- Streptavidin-HRP
- Sample Diluent
- Stop Solution (1M Phosphoric acid)
Alternate Names for Mouse IL-17/IL-17A ELISA Kit (Colorimetric)
- CTLA-8
- CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
- Cytotoxic T-lymphocyte-associated antigen 8
- IL17
- IL-17
- IL17A
- IL-17A
- IL-17Acytotoxic T-lymphocyte-associated protein 8
- IL-17CTLA-8
- IL17interleukin-17A
- interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
- interleukin 17A
Background
Mouse IL-17 mRNA is specifically expressed by activated alpha beta TCR + CD2-CD8-T-cells, a small subset with a potentially important role in immune regulation. IL-17 induces the secretion of IL-6, IL-8, PGE2, MCP-1 and G-CSF by adherent cells like fibroblasts, keratinocytes, epithelial and endothelial cells. IL-17 is also able to induce ICAM-1 surface expression of T-cells. IL-17 has been shown to support the growth of hemopoietic progenitors, it has stimulatory effects on granulopoiesis in mice. IL-17 may affect osteoclastic resorption and thereby mediate bone destruction accompanying some inflammatory diseases. Astrocytes produce NO when stimulated by IL-17, which suggests a possible role for IL-17 in the inflammatory diseases of the CNS. Forced expression of murine IL-17 induces immune responses and multiorgan inflammation. Interleukin-17 is involved in the regulation of hematopoiesis and its effects are dependent on the physiological/pathological status of the organism. IL-17 expression in airways is upregulated upon allergen exposure like in allergic asthma. Its crucial role in T-cell activation suggests that IL-17 is involved in the development of autoimmune arthritis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.