Mouse GM-CSF ELISA Kit (Colorimetric) Summary
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a mouse GM-CSF positive serum. There was no crossreactivity detected.
3.9 – 250 pg/mL
2.0 pg/mL
Sandwich ELISA
10%
5.0%
The overall mean recovery was 85%. Please see protocol for more specific Spike Recovery details.
50 uL
ELISA Kit (Colorimetric)
CSF2
Applications/Dilutions
The mouse GM-CSF ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse GM-CSF.
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
Kit Components
- Mouse GM-CSF Standard lyophilized, 500 pg/ml upon reconstitution
- Biotin-Conjugate anti-mouse GM-CSF monoclonal antibody
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Adhesive Films
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse GM-CSF
- Stop Solution (1M Phosphoric acid)
- Sample Diluent
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
- Streptavidin-HRP
- Substrate Solution (tetramethyl-benzidine)
Alternate Names for Mouse GM-CSF ELISA Kit (Colorimetric)
- colony stimulating factor 2 (granulocyte-macrophage)
- Colony-stimulating factor
- CSF
- CSF2
- GMCSF
- GM-CSF
- GMCSFgranulocyte-macrophage colony-stimulating factor
- granulocyte-macrophage colony stimulating factor
- MGC131935
- MGC138897
- molgramostin
- sargramostim
Background
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that has been characterized as a molecule capable of stimulating proliferation and differentiation of granulocyte/macrophage progenitor cells as well as modulating the functional activity of mature granulocytes and macrophages. In combination with erythropoietin, it supports the development of erythroid progenitors in vitro. The biological activities of GM-CSF substantially overlap with the effects of Interleukin 3. The reason for the redundancy of these pleiotropic cytokines may at least in part be explained by their competition for binding to common receptors. GM-CSF is produced by a number of different cell types such as activated T-cells, B-cells, macrophages, mast cells, endothelial cells, fibroblasts, synoviocytes and various tumor cells in response to various stimuli. Mouse GM-CSF as well as its receptors have been cloned. The encoded glycoprotein consists of 141 amino acid residues and has a molecular weight of about 23 kDa. The murine protein binds to a high affinity receptor complex consisting of two membrane glycoproteins, the GM-CSF receptor alpha subunit with notable homology to the human receptor and the beta subunit which also forms part of the receptors for IL-3 and IL-5. Elevated levels of GM-CSF are found in mice after endotoxin injection.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.