MITF Overexpression Lysate (Denatured) Summary

journal.pone.0136759

MITF Overexpression Lysate (Denatured) Summary

    Description
    Pasmid: pCMV-MITF full-length
    Gene
    MITF

Packaging, Storage & Formulations

    Storage
    Store at -80C. Avoid freeze-thaw cycles.
    Buffer
    Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue).

Lysate Details for MITF

    Type
    Overexpression
    Protein State
    Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-MITF antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MITF Overexpression Lysate (Denatured)

      bHLHe32
      bHLHe32MI
      Class E basic helix-loop-helix protein 32
      MI
      microphthalmia-associated transcription factor
      MITF
      Waardenburg syndrome, type 2A
      WS2A

Background

This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]. Transcript Variant: This variant (5) differs in the 5 UTR and the 5 coding region, compared to variant 1. The resulting isoform (5), contains a distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

ABT-335