MHC Class II Antibody (HIS19) [FITC] Summary
The immunogen for this antibody is MHC Class II.
IgG1
Monoclonal
Mouse
HLA-DMA
Protein A or G purified
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Applications/Dilutions
- Flow Cytometry 1:10-1:1000
The HIS19 antibody has been tested by flow cytometric analysis of rat spleen or lymph node cell suspensions. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This HIS19 clone has also been reported for use in immunoprecipitation and immunohistochemical staining of frozen tissue sections. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Reactivity Notes
Rat
Packaging, Storage & Formulations
Store at 4C in the dark.
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified
Alternate Names for MHC Class II Antibody (HIS19) [FITC]
- class II histocompatibility antigen, M alpha chain
- D6S222E
- DMA
- HLA class II histocompatibility antigen, DM alpha chain
- major histocompatibility complex, class II, DM alpha
- MHC class II antigen DMA
- Really interesting new gene 6 protein
- RING6HLADM
Background
The HIS19 monoclonal antibody reacts with a polymorphic epitope of the rat MHC class II molecule, RT1B. This antibody reacts with all rat haplotypes except the RT1n (including BN and MAXX strains). The rat MHC class II (I-A equivalent), RT1B, is expressed by antigen presenting cells including B cells, dendritic cells and macrophages.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.