j.psychres.2008.02.008

IL-13 Antibody (PVM13-1) Summary

    Immunogen
    The immunogen for this antibody was IL13.
    Isotype
    IgG1
    Clonality
    Monoclonal
    Host
    Mouse
    Gene
    IL13
    Purity
    Protein A or G purified
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Applications/Dilutions

    Dilutions
        Flow Cytometry 1:10-1:1000
        ELISA Capture (Matched Antibody Pair) 1-4ug/ml
    Application Notes
    PVM13-1 has been reported for use in intracellular flow cytometric analysis, cytokine neutralization, and ELISA. (Fluorochrome conjugated PVM13-1 is recommended for use in intracellular flow cytometry.) The PVM13-1 antibody has been tested as the capture antibody in a sandwich ELISA and ELISPOT assay for analysis of human interleukin-13 (IL-13) in combination with the biotinylated anti-human IL-13 polyclonal antibody antibody for detection and recombinant human IL-13 as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 ug/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL – 8 pg/mL should be included in each ELISA plate.

Reactivity Notes

This antibody is reactive to Human.

Packaging, Storage & Formulations

    Storage
    Store at 4C. Do not freeze.
    Buffer
    Aqueous, may contain carrier protein/stabilizer.
    Preservative
    0.09% Sodium Azide
    Concentration
    0.5 mg/ml
    Purity
    Protein A or G purified

Alternate Names for IL-13 Antibody (PVM13-1)

      ALRHMGC116789
      BHR1interleukin-13
      IL13
      IL-13
      IL-13MGC116788
      interleukin 13
      MGC116786
      NC30
      P600

Background

The PVM13-1 antibody reacts with human interleukin-13 (IL-13). IL-13, also known as NC30, is a 12.5 kDa protein secreted by activated T helper cells, CD8+ T cells, and NK cells. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression and suppression of inflammatory cytokine expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

SB-431543

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