IL-13 Antibody (32116.11) Summary
Recombinant full length human IL13 protein expressed in E. coli.
Secreted
Recognizes recombinant human IL-13 by capture ELISA and neutralization.
IgG1
Monoclonal
Mouse
IL13
Protein A purified
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Applications/Dilutions
- Western Blot 1-2 ug/ml
- Blocking/Neutralizing 1-3 ug/ml
- ELISA 4 ug/ml
ELISA: Use in combination with a biotinylated IL13 polyclonal detection antibody at 400 ng/ml (100 ul/well), an ELISA for sample volumes of 100 ul with a range of 62.5-4000 ug/ml can be obtained. Neut: Use at a concentration of 1-3 ug/ml in the presence of 10 ng/ml of recombinant human IL13 using the human erythroleukemia cell line, TF-1. WB: Detects a band of approximately 9 kDa (predicted molecular weight: 12 kDa). The detection limit for recombinant human IL13 is approximately 50 ng/lane under non-reducing and reducing conditions. Endotoxin level is < 10 ng/mg antibody as determined by the LAL (Limulus amebocyte lysate) method. Optimal dilutions/concentrations should be determined by the end user.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 5% Trehalose
No Preservative
0.5 mg/ml
Protein A purified
Alternate Names for IL-13 Antibody (32116.11)
- ALRHMGC116789
- BHR1interleukin-13
- IL13
- IL-13
- IL-13MGC116788
- interleukin 13
- MGC116786
- NC30
- P600
Background
Human IL-13 was originally identified by differential screening of an anti-CD28 activated human peripheral blood mononuclear cell cDNA library as an induction-specific novel cytokine. It was also isolated from cDNA libraries of human T cell clones using the murine IL-13 (P600) cDNA as a probe.2 Human IL-13, a pleiotropic cytokine, is produced by activated Th0, Th1-like, Th2-like, and CD8 T cells.1 The gene for human IL-13 maps to chromosome 5 and is closely linked to the genes for IL-3, IL-4, IL-5, and GM-CSF.3 IL-13 inhibits proinflammatory cytokine production and stimulates antibody production.1 It induces proliferation in the human pre-myeloid cell line TF-1. IL-13 has multiple effects on the differentiation and functions of monocytes and macrophages. It suppresses cytotoxic functions and induces changes in the morphology of human monocytes and in the phenotype of human monocytes and B cells by upregulating MHC class II expression.4 IL-13 will also decrease the production of nitric oxide by activated murine macrophages, leading to impaired parasiticidal activity.5
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
product targets :　Neuropeptide Y Receptor inhibitors