IL-10 Antibody (JES5-2A5) [PE] Summary
The antibody reacts with the IL-10 antigen.
This anti‑mIL‑10 neutralizes the biological activity of murine IL‑10. It does not cross‑react with Hu‑IL‑10. Murine interleukin 10 (IL 10) is a multifunctio¬nal 30 40 kDa cytokine which is produced by TH2 mouse T Helper cell clones and which inhibits the synthesis of several cytokines by TH1 cells. Interleukin 10 was for this reason originally known as the Cytokine Synthesis Inhibitory Factor (CSIF). Interleukin 10 has activities which involve a wide variety of cell types. These activities include: inhibition of macrophage cytokine synthesis; inhibition of antigen presentation (selective for TH1 cells); enhancement of B cell survival; enhancement of MHC class II expression; contribution to the overall function of TH2 cells. Murine IL 10 has been shown to exhibit extensive sequence similarity to Human IL 10 and to an open reading frame in the Epstein ¬Barr virus genome BLRF 1. In contrast to Human IL 10 which is active on murine cells, murine IL 10 is not active in human cells. Given the aforementioned range of effects of IL 10 and its potential anti in¬flamma¬tory prop¬erties, antibodies capable of blocking the biologi¬cal activity are clearly essential in the study of these effects.
IgG1
Monoclonal
Rat
IL10
Protein G purified
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Applications/Dilutions
- Flow Cytometry Neat
Reactivity Notes
Human,Mouse
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
PBS, 0.5% BSA, and 5% Sucrose
0.05% Sodium Azide
Protein G purified
Alternate Names for IL-10 Antibody (JES5-2A5) [PE]
- CSIF
- CSIFMGC126450
- Cytokine synthesis inhibitory factor
- IL10
- IL-10
- IL10A
- IL-10MGC126451
- interleukin 10
- interleukin-10
- TGIF
Background
Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 160 amino acids. There is 73% homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.