cmaj.1040482

IGF-II Antibody Summary

    Immunogen
    Purified recombinant mouse IGF2 protein
    Localization
    Secreted protein
    Specificity
    Detects a band at approximately 20kDa. Based on ELISA, this has a 25% cross-reactivity to human IGF2.
    Isotype
    IgG
    Clonality
    Polyclonal
    Host
    Goat
    Gene
    IGF2
    Purity
    Immunogen affinity purified
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Applications/Dilutions

    Dilutions
        Immunohistochemistry 1:10-1:500
        Immunohistochemistry-Paraffin 1:10-1:500
    Positive Control MCF-7 Lysate (NB820-59465)

Reactivity Notes

Mouse.

Packaging, Storage & Formulations

    Storage
    Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
    Buffer
    PBS and 5% Trehalose
    Preservative
    No Preservative
    Concentration
    0.1 mg/ml
    Purity
    Immunogen affinity purified

Alternate Names for IGF-II Antibody

      C11orf43
      chromosome 11 open reading frame 43
      FLJ22066
      FLJ44734
      IGF2
      IGF-2
      IGFII
      IGF-II
      insulin-like growth factor 2 (somatomedin A)
      insulin-like growth factor II
      insulin-like growth factor type 2
      MSA
      PP9974
      somatomedin-A

Background

Insulin-like growth factor II (also known as multiplication stimulating activity or MSA) and insulin-like growth factor I (IGF-I) belong to the family of insulin-like growth factors which are structurally homologous to proinsulin. Mature IGF-I and IGF-II are highly conserved and share approx imately 70% amino acid sequence identity. Mouse IGF-II, a 67 amino acid protein, has a predicted molecular mass of approximately 7.4 kDa. Mouse and human IGF-II share 91% sequence identity. Insulin-like growth factor II has autocrine, paracrine, and endocrine functions. It is a potent mitogenic growth factor that mediates growth-promoting activities in embryonic development. IGF-II binds the IGF-II receptor with high affinity. IGF-I and IGF II are expressed in many tissues and cell types. IGF-II is mitogenic for a variety of cultured cells including human or chicken fibroblasts, mouse 3T3 cells, normal rat kidney cells, and MCF-7 human breast carcinoma cells.2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

product targets : MAPK_Compound_Library inhibitors

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