toxins8010002

Human TOR/mTOR ELISA Kit (Colorimetric) Summary

    Description
    Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. mTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). Novus mTOR ELISA kit is a sandwich ELISA assay for the quantitative measurement of human mTOR in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of mTOR captured from the samples.
    Standard Curve Range
    0.156 – 10 ng/ml
    Sensitivity
    < 0.094 ng/ml
    Assay Type
    Sandwich ELISA
    Sample Volume
    0.1 ml
    Kit Type
    ELISA Kit (Colorimetric)
    Gene
    MTOR

Applications/Dilutions

    Application Notes
    Detection Method: Absorbance (450 nm). Quantitative measurement of mTOR in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.

Packaging, Storage & Formulations

    Storage
    Store at -20C.

Kit Components

Components
      TMB substrate
      Wash buffer (25X)
      Stop Solution
      SABC dilution buffer
      Sample / Standard dilution buffer
      Biotin- detection antibody (Concentrated)
      Antibody dilution buffer
      HRP-Streptavidin Conjugate (SABC)
      Lyophilized Standard
      Microplate: 96 wells
      Plate sealers

Alternate Names for Human TOR/mTOR ELISA Kit (Colorimetric)

      EC 2.7.11.1
      FK506 binding protein 12-rapamycin associated protein 1
      FK506 binding protein 12-rapamycin associated protein 2
      FK506-binding protein 12-rapamycin complex-associated protein 1
      FKBP12-rapamycin complex-associated protein
      FLJ44809
      FRAP
      FRAP1
      FRAP1FKBP12-rapamycin complex-associated protein 1
      FRAP2
      Mammalian target of rapamycin
      mechanistic target of rapamycin (serine/threonine kinase)
      Mechanistic target of rapamycin
      MTOR
      RAFT1
      rapamycin and FKBP12 target 1
      rapamycin associated protein FRAP2
      Rapamycin target protein 1
      RAPT1FKBP-rapamycin associated protein
      serine/threonine-protein kinase mTOR
      TOR

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

STF-118805

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