rspb.2003.2581

Human TIMP-1 ELISA Kit (Colorimetric) Summary

    Standard Curve Range
    39 – 2500 pg/mL
    Sensitivity
    10.0 pg/mL
    Assay Type
    Sandwich ELISA
    Kit Type
    ELISA Kit (Colorimetric)
    Gene
    TIMP1

Applications/Dilutions

    Application Notes
    This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Reactivity Notes

Human

Packaging, Storage & Formulations

    Storage
    Storage of components varies. See protocol for specific instructions.

Kit Components

Components
      Stop Solution (1M Phosphoric acid)
      Substrate Solution (tetramethyl-benzidine)
      Streptavidin-HRP
      Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
      Biotin-Conjugate anti-human TIMP-1 polyclonal antibody
      Human TIMP-1 Standard lyophilized, 5 ng/ml upon reconstitution
      Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
      Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human TIMP-1
      Adhesive Films

Alternate Names for Human TIMP-1 ELISA Kit (Colorimetric)

      CLGI
      Collagenase inhibitor
      collagenase inhibitor)
      EPATIMP-1
      EPO
      erythroid potentiating activity
      Erythroid-potentiating activity
      Fibroblast collagenase inhibitor
      FLJ90373
      HCI
      metalloproteinase inhibitor 1
      TIMP metallopeptidase inhibitor 1
      TIMP1
      TIMP-1
      TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity
      Tissue inhibitor of metalloproteinases 1

Background

The tissue inhibitions of metalloproteinases (TIMPs) are naturally occurring proteins that specifically inhibit matrix metalloproteinases, thus maintaining balance between matrix destruction and formation. An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. TIMP-1 has been shown to inhibit tumor-induced angiogenesis. An imbalance of MMP/TIMP regulation has been implicated in several inflammatory diseases of the nervous system. In the pathogenesis of vascular diseases TIMP-1 seems to be down regulated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

SP600126

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