Human TGF-beta 1 ELISA Kit (Colorimetric) Summary
The assay detects both natural and recombinant human TGF beta 1. The cross reactivity of TGF beta 2 and TGF beta 3, and of TNF beta, IL- 8, IL-6, IL-2, TNF alpha, IL-1 beta, IL-4, IFN gamma, IL-12p70, IL-5 and IL-10 was evaluated by spiking these proteins at physiologically relevant concentrations into serum. There was no cross reactivity detected.
31.3 – 2,000 pg/mL
9.0 pg/mL
Sandwich ELISA
4.90%
3.20%
Please see protocol for more specific Spike Recovery details.
40 uL (pretreated)
ELISA Kit (Colorimetric)
TGFB1
Applications/Dilutions
The human TGF-beta1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human TGF-beta1.
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
Kit Components
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human TGF-beta1
- Adhesive Films
- Human TGF-beta1 Standard lyophilized, 4 ng/mL upon reconstitution
- Biotin-Conjugate anti-human TGF-beta1 monoclonal antibody
- Stop Solution (1M Phosphoric acid)
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
- Substrate Solution (tetramethyl-benzidine)
- Streptavidin-HRP
Alternate Names for Human TGF-beta 1 ELISA Kit (Colorimetric)
- CEDLAP
- DPD1
- latency-associated peptide
- TGFB
- TGFB1
- TGF-beta 1 protein
- TGFbeta 1
- TGF-beta 1
- TGFbeta
- TGF-beta-1
- transforming growth factor beta-1
- transforming growth factor, beta 1
Background
TGF-beta plays a critical role in cellular growth, development, differentiation, proliferation, extracellular matrix (ECM) synthesis and degradation, control of mesenchymal-epithelial interactions during embryogenesis, immune modulation, apoptosis, cell cycle progression, angiogenesis, adhesion and migration and leukocyte chemotaxis. It has both tumor suppressive and tumor promoting activities and is highly regulated at all levels. TGF-beta1 is the key mediator in the pathophysiology of tissue repair and human fibrogenesis: balance between production and degradation of type I collagen, and fibrosis and scarring in organ and tissue. TGF-beta1 promotes Treg cell differentiation resulting in IL-10/TGF-beta1 production and Th1 cell and Th2 cell suppression.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.