Human MMP-9 ELISA Kit (Colorimetric) Summary
The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human MMP-9 positive sample. There was no cross reactivity or interference detected.
0.23 – 15.0 ng/mL
0.05 ng/mL
Sandwich ELISA
10.2%
7.3%
Recoveries were determined in 2 independent experiments with 4 replicates each. The overall mean recovery was103.1%.
10 uL (prediluted 1 : 25)
ELISA Kit (Colorimetric)
MMP9
Applications/Dilutions
This kit is an enzyme-linked immunosorbent assay for quantitative detection.
Reactivity Notes
Human
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
Kit Components
- Stop Solution (1M Phosphoric acid)
- Substrate Solution (tetramethyl-benzidine)
- Streptavidin-HRP
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
- Biotin-Conjugate anti-human MMP-9 polyclonal antibody
- Human MMP-9 Standard lyophilized, 30 ng/ml upon reconstitution
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Adhesive Films
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human MMP-9
Alternate Names for Human MMP-9 ELISA Kit (Colorimetric)
- 92 kDa gelatinase
- 92 kDa type IV collagenase
- CLG4B
- EC 3.4.24
- EC 3.4.24.35
- Gelatinase B
- GELB
- macrophage gelatinase
- MANDP2
- matrix metallopeptidase 9
- matrix metalloproteinase 9
- matrix metalloproteinase-9
- MMP9
- MMP-9
- type V collagenase
Background
MMP-9 is a Zn+2 dependent endopeptidase, synthesized and secreted in monomeric form as zymogen. The structure is almost similar to MMP2, another member of matrixmetalloproteinase family. Like other proteolytic enzymes, MMP-9 is first synthesized as inactive proenzyme or zymogens. Activation of proMMP-9 is mediated by plasminogen activator/plasmin (PA/plasmin) system. The regulation of MMP-9 activity is also controlled through TIMP-3. MMP-9 plays an important role in tumor invasion, angiogenesis and metastasis. Increased levels of MMP-9 have been demonstrated in colorectal cancer, in acute leukemia, breast cancer, human melanoma and bladder cancer. MMP-9 potentiates pulmonary metastasis formation and glioma invasion. Apart from its function in tumor formation and spread, MMP-9 with its destructive effects is in close association with lung diseases and asthma. Leukocyte recruitment to the central nervous system MMP-9 means an involvement in the pathogenesis of multiple sclerosis. MMP-9 has a crucial role in reproductive endocrinology and shows a changed expression pattern after myocardial infarction.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.