Human IL-5 ELISA Kit (Colorimetric) Summary
7.8 – 500 pg/mL
1.5 pg/mL
Sandwich ELISA
6.80%
6.60%
74%
50 uL
ELISA Kit (Colorimetric)
IL5
Applications/Dilutions
The human IL-5 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-5.
Reactivity Notes
Human
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
Kit Components
- Aluminium pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to human IL-5, Biotin-Conjugate (anti-IL-5 monoclonal antibody), Streptavidin-HRP and Sample Diluent, lyophilized
- Adhesive Plate Covers
- Aluminium pouch(es) with a human IL-5 Standard curve (coloured)
- Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
- Substrate Solution (tetramethyl-benzidine)
- Sample Diluent (Use when an external predilution of the samples is needed)
- Stop Solution (1M Phosphoric acid)
Alternate Names for Human IL-5 ELISA Kit (Colorimetric)
- BCDF mu
- B-cell differentiation factor I
- BCGFII
- EDF
- Eo-CSF
- Eosinophil differentiation factor
- IL5
- IL-5
- IL-5T-cell replacing factor
- interleukin 5 (colony-stimulating factor, eosinophil)
- interleukin-5
- TRF
- TRFB cell differentiation factor I
Background
The human IL-5 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-5. Human IL-5 has been described as T-cell replacing factor and as B-cell growth factor II based on its induction of proliferation and Ig secretion by activated B cells. Several other activities have furthermore been attributed to this cytokine, including induction of growth and differentiation of eosinophils from bone marrow stem cells, induction of mIL-2R expression on activated B cells and enhancements of IgA secretion by LPS-stimulated B-cells. IL-5 has been shown to be produced by the TH2 subset of human TH clones. The spectrum of biological activities of IL-5, on B cells as well as other cell types suggests that IL-5 plays an important functional role in the activities of TH2 cells. IL-5 is a heavily glycosylated glycoprotein.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.